進一步提高血清促甲狀腺激素（TSH）檢測的敏感性
增強化學發(fā)光酶免疫法測定血清促甲狀腺激素方法的建立及初步應用
通過正交試驗，并經(jīng)方差分析，獲得增強發(fā)光酶免疫檢測法的*實驗條件，將其與TSH-酶聯(lián)免疫吸附測定（ELISA）試劑盒相結(jié)合，建立TSH的增強化學發(fā)光酶免疫檢測法
To improve the sensitivity of measurement of TSH in serum.Methods Establishment and application of a new chemiluminescence enzyme immunoassay to determine human thyrotropin in serum LIANG Yan，LIN Hanhua，WANG Muti. Department of Pediatrics, Tongji Hospital,Tongji Medical University
The authors developed a new enhanced chemiluminescence enzyme immunoassay （ECLIA）, which combined luminol-H2O2-HRP system enhanced by para-iodophenol（PIP） with TSH-ELISA test kit. The optimal experimental conditions of the enhanced chemiluminescence were obtained by orthogonal design and analysis of variance
材料和方法
儀器和試劑：Metrohm Herisau E350B-pH計（瑞士）。魯米諾 （Luminol,Serva） 用pH 10.3、0.05 mol/L 碳酸鹽緩沖液配制成5×10-2 mol/L儲備液放置3天后再以pH 8.2、0.1 mol/L 碳酸氫鈉-氫氧化鈉緩沖液稀釋備用。
將化學發(fā)光技術(shù)與TSH-ELISA試劑盒相結(jié)合，建立了TSH的增強化學發(fā)光酶免疫檢測法
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